活力/细胞毒性测定试剂盒

用于动物活细胞和死细胞的活力/细胞毒性检测试剂盒用绿色荧光染色活细胞，用红色荧光染色死细胞，以便通过荧光显微镜或流式细胞术检测。Calcein-AM 是一种非荧光细胞渗透性酯，可以被动进入细胞。在活细胞中，它被细胞质中的酯酶水解，释放出绿色荧光染料钙黄绿素。钙黄绿素本身是膜不可渗透的，仅保留在具有完整质膜的活细胞中。Ethidium Homodimer III 是一种红色荧光不透膜核酸染料。它选择性地染色质膜受损的死细胞（坏死或晚期凋亡细胞）。

细胞毒性/活力测定	目录号	尺寸
Viability/Cytotoxicity Assay for Animal Live & Dead Cells	30002-T	150 次检测
Viability/Cytotoxicity Assay for Animal Live & Dead Cells	30002	300 次检测

HeLa 细胞用动物活细胞和死细胞的活力/细胞毒性测定法染色。死细胞被染成红色，活细胞被染成绿色。

死细胞核酸染色

膜不透性核酸染料可用于选择性地染色具有渗漏质膜的坏死和晚期凋亡细胞。这些染料在结合 DNA 或 RNA 之前具有非常低的荧光，因此它们可用于染色细胞而无需洗涤步骤，以便在细胞培养基中进行均质和实时成像。Biotium 提供多种颜色的死细胞染色剂，以及带有死细胞染色剂和凋亡标记物的细胞凋亡和坏死染色试剂盒。

In general, dead cell nucleic acid dyes cannot be fixed after staining, because the dyes do not become cross-linked to DNA. After fixation, they are free to diffuse from dead cells to live cells (which become permeabilized during fixation), resulting in poor discrimination between live and dead cell populations. For fixable dead cell stains, see our Live-or-Dye™ product line, including the nuclear-specific Live-or-Dye NucFix™ Red.

Dead Cell Nucleic Acid Stains	Catalog No.	*Color (Ex/Em)**	Features
Oxazole Blue, 1 mM in DMSO	40091	Blue (434/457 nm)	• Blue cell impermeant dye • Selectively stains early apoptotic cells • Equivalent to PO-PRO™-1 Iodide
Oxazole Blue Homodimer, 1 mM in DMSO	40093	Blue (433/457 nm)	• Blue cell impermeant dye • Equivalent to POPO™-1 Iodide
NucSpot® 470, 1000X in DMSO	40083	Green (460/546 nm)	• Green cell impermeant dye • Nuclear-specific counterstain in fixed cells • Useful for cell cycle analysis in fixed cells • Excellent match for blue LED excitation sources
Oxazole Yellow, 1 mM in DMSO	40089	Green (491/506 nm)	• Green cell impermeant dye • Selectively stains early apoptotic cells • Equivalent to YO-PRO®-1 Iodide
Oxazole Yellow Homodimer, 1 mM in DMSO	40090	Green (491/508 nm)	• Green cell impermeant dye • Equivalent to YOYO®-1 Iodide
TO Iodide, 1 mM in DMSO	40088	Green (515/531 nm)	• Green cell impermeant dye • Equivalent to TO-PRO®-1 Iodide
Thiazole Orange Homodimer, 1 mM in DMSO	40079	Green (514/531 nm)	• High affinity dimeric cyanine dye • Dead cell stain and electrophoresis dye • Equivalent to TOTO®-1 Iodide
NucSpot® 555/570 Nuclear Stain	41033	Red (559/566 nm)	• Red cell impermeant dye for the Cy®3 or PE channels • Nuclear-specific counterstain in fixed cells
NucSpot® 568/580 Nuclear Stain	41036	Red (572/583 nm)	• Red cell impermeant dye for the Cy®3 or PE channels • Nuclear-specific counterstain in fixed cells • Suitable for multi-day live cell imaging
NucSpot® 594/615 Nuclear Stain	41037	Red (603/613 nm)	• Red cell impermeant dye for the Texas Red® or PE-Texas Red® channels • Nuclear-specific counterstain in fixed cells • Suitable for multi-day live cell imaging
Propidium Iodide, 100 mg	40016	Red (530/622 nm)	• Widely used dead cell stain • Can be excited by 488 nm laser line for detection in the PE channel by flow cytometry • Useful for cell cycle analysis in fixed cells (with RNase treatment)
Propidium Iodide, 1 mg/mL in Water	40017
Propidium Iodide Buffer, 50 ug/mL	40048
Ethidium Homodimer I, 1 mg	40010	Red (527/624 nm)	• High-affinity membrane-impermeant nucleic acid stain • >30-fold fluorescence enhancement upon binding to DNA/RNA • High-purity grade not available from other manufacturers
Ethidium Homodimer I, 2 mM in DMSO	40014	Red (527/624 nm)
Ethidium Homodimer III, 1 mg	40050	Red (532/625 nm)	• Developed at Biotium as an alternative to Ethidium Homodimer I • 45% brighter than EthDI when bound to DNA
Ethidium Homodimer III, 1 mM in DMSO	40051	Red (532/625 nm)
7-AAD, 1 mg	40037	Far-red (546/647 nm)	• Far-red dye for flow cytometry detection in the PE-Cy®5 channel • Can be excited by the 488 nm or 532 nm laser line • Useful for cell cycle analysis in fixed cells
7-AAD, 1 mg/mL solution	40084	Far-red (546/647 nm)
NucSpot® Far-Red, 1000X in DMSO	40085	Far-red (597/667 nm)	• Designed as improved replacement for 7-AAD • For flow cytometry in the PE-Cy®5 or APC channel • Useful for cell cycle analysis in fixed cells • Less bleed into the PE-Texas Red® channel
RedDot™2 Far-Red Nuclear Stain	40061	Far-red (665/695 nm)	• Far-red cell impermeant dye for the Cy®5 channel • Nuclear-specific counterstain in fixed cells • Replaces Draq7™
Thiazole Red, 1 mM in DMSO	40087	Far-red (642/657 nm)	• Far-red cell impermeant dye for the Cy®5 channel • Dead cell stain and electrophoresis dye • Equivalent to TO-PRO®-3 Iodide
Thiazole Red Homodimer, 1 mM in DMSO	40080	Far-red (642/661 nm)	• High affinity dimeric cyanine dye for the Cy®5 channel • Useful dead cell stain • Equivalent to TOTO®-3 Iodide
NucSpot® 650/665 Nuclear Stain	41034	Far-red (653/671 nm)	• Far-red cell impermeant dye for the Cy®5 or APC channels • Nuclear-specific counterstain in fixed cells
NucSpot® 680/700 Nuclear Stain	41035	Near-IR (683/707 nm)	• Near-IR cell impermeant dye for the Cy®5.5 channel • Nuclear-specific counterstain in fixed cells
NucSpot® 750/780 Nuclear Stain	41038	Near-IR (757/780 nm)	• Near-IR cell impermeant dye for the Cy®7 or APC-Cy®7 channels • Nuclear-specific counterstain in fixed cells • Suitable for multi-day live cell imaging

* 当与核酸结合时，
SYBR、PO-PRO、POPO、Texas Red、TOTO、TO-PRO、YO-PRO 和 YOYO 是 Thermo Fisher Scientific 的商标或注册商标。Cy Dye 是 Cytiva 的注册商标。Draq7 是 Biostatus Ltd 的商标。

Biotium 为酶标仪、流式细胞术或荧光显微镜提供多种细胞活力和细胞死亡检测试剂盒。跳转到下面的部分以了解更多信息：