Mix-n-Stain™ 荧光蛋白和串联染料抗体标记试剂盒可让您在大约 4 小时内用荧光藻胆蛋白标记您的抗体，无需纯化。标记程序可容忍许多常见的缓冲液成分和抗体稳定剂（详情请参阅产品协议）。

• APC、R-PE (PE)、PerCP 或串联染料的选择

• 贴标签需要几个小时，而且手动操作时间最少

• 用于标记 25-50 ug、50-100 ug 或 1 mg IgG 的试剂盒规格

• 无需纯化，100%回收

荧光蛋白（APC、R-PE 和 PerCP）：

• APC（别藻蓝蛋白）是一种约 105 kDa 的明亮藻胆蛋白，存在于红藻和蓝藻（蓝绿藻）中。它在 650 nm 处具有吸收最大值，在 660 nm 处具有发射最大值。由于荧光量子产率高 ( ~ 0.68)，APC 是迄今为止 650 nm 波长区域最亮的染料。

• R-PE (R-phycoerythrin, also called PE) is a large phycobiliprotein with a molecular weight of ~240 kDa. It can be excited by 488, 532, and 561 nm laser lines and has an emission maxima at 576 nm. Because of the high extinction coefficient (~1,960,000) and high fluorescence quantum yield (~0.82), R-PE is by far the brightest dye for the 565 nm wavelength region. R-PE is one of the brightest fluorochromes for flow cytometry, but due to its photobleaching properties it is not recommended for fluorescence microscopy.

• PerCP (Peridinin Chlorophyll Protein Complex) is a small, 35 kDa, phycobiliprotein found in dinoflagellates. PerCP has absorption maxima at 482 nm and emission at 677 nm.

Tandem dyes:

Tandem dyes are composed of two covalently attached fluorescent molecules. An example of this a phycobiliprotein to which small molecule dyes are conjugated. By a process called Förster resonance energy transfer (FRET), or fluorescence resonance energy transfer, the tandem dye has the excitation properties of the donor molecule and the emission properties of the acceptor. The Stokes shift of the tandem dye is larger than that of the original donor molecule. Tandem dyes are used almost exclusively for flow cytometry because of imperfect energy transfer, and because they show bleed-through fluorescence in the acceptor dye channel, which requires compensation. Aside from cytometers, most fluorescence instruments don’t perform compensation and are generally not configured for the long-stokes shifts of tandem dyes.

我们提供使用以下串联染料进行标记的试剂盒，这些染料具有 Biotium 的下一代CF® 染料，具有出色的亮度。

• RPE-CF®583R（Ex/Em 488、532、561/608 nm）用于 RPE-Texas Red® 通道

• RPE-CF®647T（Ex/Em 488、532、561/673 nm）用于 RPE-Cy®5 通道

• APC-CF®750T (Ex/Em 633, 640/785 nm) 用于 APC-Cy®7 通道

• APC-CF®790T (Ex/Em 633, 640/814 nm) 用于 APC-Cy®7 通道

注意： APC-CF®790T 适用于光谱流式细胞术。对于传统的流式细胞术，请使用 APC-CF®750T。

我们还提供 Mix-n-Stain™ 试剂盒，用于使用明亮且光稳定的 CF® 染料和其他标签标记抗体、纳米抗体或小配体。查看我们全系列的抗体和蛋白质标记试剂盒。

混合染色™ 荧光蛋白抗体标记试剂盒

注意：不推荐使用试剂盒标记 IgM 抗体。Cy Dye 是 Cytiva 的注册商标。